NLS-Cre Recombinase mRNA encodes a codon-optimized Cre recombinase variant fused to a nuclear localization sequence (NLS). Derived from the P1 bacteriophage, Cre recombinase catalyzes site-specific recombination between loxP sites. Optimized for mammalian systems
- mRNA lenght : 1533 nts
- Concentration : 1mg/ml
- Buffer: 1 mM sodium citrate, pH 6.4
PureBoost™ Technology
Cellerna mRNAs are produced via PureBoost™ technology which maximizes protein expression and minimizes mRNA immunogenicity. PureBoost™ is a proprietary technology which combines novel advanced capping and ultra-purification processes of in vitro-transcribed mRNAs that have been optimized for robust protein expression. Uniform polyadenylation (110 bp polyA-tail) and almost perfect capping (Cap1 > 98%) mimic natural mRNA and ensure efficient translation. Sequence engineering or chemical modification reduces the innate antiviral response to single-stranded mRNA.
PureBoost™ Cre mRNA
- Gene Name: NLS-Cre Recombinase
- Species: Various, synthetic sequence
- Encoded protein: Cre recombinase, fused to a nuclear localization sequence (NLS)
- Protein Sequence
- Codon Optimization: Yes
- Application: CRISPR, Immunotherapeutics, Recombinases