The PureBoost™ EGFP mRNA contains a codon-optimized nucleotide sequence of the green fluorescent protein, which was originally isolated from the jellyfish, Aequorea victoria. EGFP is a direct detection reporter gene commonly used in mammalian cell cultures to produce bright green fluorescence with an emission peak at 509 nm. It is commonly used as a positive control for mRNA transfections and for transient labeling of cells by transfection.
- mRNA lenght : 1179 nts
- Concentration : 1mg/ml
- Buffer: 1 mM sodium citrate, pH 6.4
PureBoost™ Technology
Cellerna mRNAs are produced via PureBoost™ technology which maximizes protein expression and minimizes mRNA immunogenicity. PureBoost™ is a proprietary technology which combines novel advanced capping and ultra-purification processes of in vitro-transcribed mRNAs that have been optimized for robust protein expression. Uniform polyadenylation (110 bp polyA-tail) and almost perfect capping (Cap1 > 98%) mimic natural mRNA and ensure efficient translation. Sequence engineering or chemical modification reduces the innate antiviral response to single-stranded mRNA.
PureBoost™ EGFP mRNA
- Gene Name : EGFP
- Species: Aequorea victoria.
- Encoded protein: EGFP
- Function : Reporter
- Protein Sequence
- Codon Optimization: Yes
- Application: Positive control for mRNA transfections